Minggu, 05 September 2021

Analysis of APXs and HSPs genes responsible to respond to heat stress in tomato plants cultivated in Central Sulawesi

Central Sulawesi is one of the areas in Indonesia located on the equator which has hot temperatures. This temperature greatly affects the growth of tomato plants. However, this plant can grow well. It is not yet known what crucial genes are responsible for the heat tolerance. Therefore, a study was conducted to reveal some genes; APX1, APX2, HSP17.4, HSP17.6, HSP70, HSP90. Those genes are responsible for the heat tolerance. The study was carried out by the PCR method to determine the APX1, APX2, HSP17.4, HSP17.6, HSP70, HSP90 genes. The DNA of these genes was isolated from the leaves and then cDNA was made to determine gene expression at the transcription level. Also, a qualitative analysis based on the NCBI search was used to describe the structure and function of each observed gene. The results showed that the genes; APX1, APX2, HSP17.4, HSP17.6, HSP70, HSP90 were expressed in different levels in tomato plants from the Central Sulawesi region. Thus, it can be suggested that these genes have an important role in heat tolerance in the tomato plant. For further go to link https://drive.google.com/file/d/13HS4KPe7Jd8v7j7ZyhR_hgP2OK_YlQCi/view?usp=sharing 

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